Ee Research Group
Pharmaceutical Science Laboratory
Ee Research Group
Pharmaceutical Science Laboratory
Publications
2025
Coming soon...
2024
Bacterial clustering biomaterials as anti-infective therapies.Tram, N. D. T., Xu, J., Chan, K. H., Rajamani, L., & Ee, P. L. R. (2024)
Peptides with antimicrobial properties. Brandon Isamu MORINAKA, Ryosuke SUGIYAMA, Ziwei YAO, Pui Lai Rachel EE, Dai Thien Nhan TRAM, Yohei Morishita, Chin-Soon PHAN, Joel LIM. (2024)
Nano Letters, 24(43), 13574-13582.
Structure-Guided Bacteria Specificity and Wide Activity Spectrum of Endotoxin-Responsive Peptide Nanonets.Tram, N. D. T., Marzinek, J. K., Perrin, L., Mukherjee, D., Selvarajan, V., Bond, P. J., & Ee, P. L. R. (2024)
2023
Advanced Functional Materials, 33(5), 2210858.
Bacteria-Responsive Self-Assembly of Antimicrobial Peptide Nanonets for Trap-and-Kill of Antibiotic-Resistant Strains.Tram, N. D. T., Xu, J., Mukherjee, D., Obanel, A. E., Mayandi, V., Selvarajan, V., ... & Ee, P. L. R. (2023)
Chemical Engineering Research and Design, 192, 477-486.
The influence of pregelatinized starch on the rheology of a gellan gum-collagen IPN hydrogel for 3D bioprinting.
Ng, J. Y., Yu, P., Murali, D. M., Liu, Y. S., Gokhale, R., & Ee, P. L. R. (2023)
Advanced Healthcare Materials, 12(20), 2203232.
Multifunctional Antibacterial Nanonets Attenuate Inflammatory Responses through Selective Trapping of Endotoxins and Pro‐Inflammatory Cytokines. Tram, N. D. T., Tran, Q. T. N., Xu, J., Su, J. C. T., Liao, W., Wong, W. S. F., & Ee, P. L. R. (2023)
Journal of Medicinal Chemistry, 66(13), 8498-8509.
Stapled β-hairpin antimicrobial peptides with improved stability and activity against drug-resistant Gram-negative bacteria.Selvarajan, V., Tram, N. D., Xu, J., Ngen, S. T., Koh, J. J., Teo, J. W., ... & Ee, P. L. R. (2023).
2022
3d-bioprinting of cell-laden-collagen gellan gum interpenetrating network hydrogel.
Pui Lai Rachel EE, Jian Yao NG, Rajeev Gokhale, Yuan Siang LUI. (2022)
Addressing antimicrobial resistance with the IDentif. AI platform: Rapidly optimizing clinically actionable combination therapy regimens against nontuberculous mycobacteria.
Mukherjee, D., Wang, P., Hooi, L., Sandhu, V., You, K., Blasiak, A., ... & Ee, P. L. R. (2022)
Biomacromolecules, 23(7), 2803-2813.
Sugar-assisted cryopreservation of stem cell-laden gellan gum–collagen interpenetrating network hydrogels. Ng, J. Y., Tan, K. Y. F., & Ee, P. L. R. (2022).
Frontiers in Bioscience-Landmark, 27(2), 64.
Novel tetrameric cell penetrating antimicrobial peptoids effective against mycobacteria and drug-resistant Staphylococcus aureus. Fleck, B. S., Mukherjee, D., Tram, N. D. T., Ee, P. L. R., & Schepers, U. (2022).
Rationalisation of Antifungal Properties of α-Helical Pore-Forming Peptide, Mastoparan B.
Lim, E. J., Leng, E. G. T., Tram, N. D. T., Periayah, M. H., Ee, P. L. R., Barkham, T. M. S., ... & Lakshminarayanan, R. (2022).
2021
Nature Biomedical Engineering, 5(10), 1217-1227.
Wirelessly operated bioelectronic sutures for the monitoring of deep surgical wounds.
Kalidasan, V., Yang, X., Xiong, Z., Li, R. R., Yao, H., Godaba, H., ... & Ho, J. S. (2021).
ACS Applied Bio Materials, 4(2), 1470-1482.
Pristine gellan gum–collagen interpenetrating network hydrogels as mechanically enhanced anti-inflammatory biologic wound dressings for burn wound therapy.
Ng, J. Y., Zhu, X., Mukherjee, D., Zhang, C., Hong, S., Kumar, Y., ... & Ee, P. L. R. (2021).
Acta Biomaterialia, 135, 214-224.
Manipulating turn residues on de novo designed β-hairpin peptides for selectivity against drug-resistant bacteria. Tram, N. D., Selvarajan, V., Boags, A., Mukherjee, D., Marzinek, J. K., Cheng, B., ... & Ee, P. L. R. (2021).
Royal Society of Chemistry, Book Chapter 9
Carbon Nanomaterials for the Development of Biosensors for Microbe Detection and Diagnosis.
Tram, N. D. T., Zhu, X., Ee, P. L. R., & Pastorin, G. (2021).
2020
Molecular biomedicine, 1(1), 11.
Developing inhaled protein therapeutics for lung diseases.
Matthews, A. A., Ee, P. L. R., & Ge, R. (2020).
Carbohydrate polymers, 241, 116345.
Biomimicry of microbial polysaccharide hydrogels for tissue engineering and regenerative medicine–A review. Ng, J. Y., Obuobi, S., Chua, M. L., Zhang, C., Hong, S., Kumar, Y., ... & Ee, P. L. R. (2020).
Frontiers in Chemistry, 8, 602.
Silica nanoparticles—a versatile tool for the treatment of bacterial infections.
Selvarajan, V., Obuobi, S., & Ee, P. L. R. (2020).
Frontiers in Bioengineering and Biotechnology, 8, 564667.
Chlorella vulgaris extract as a serum replacement that enhances mammalian cell growth and protein expression.
Ng, J. Y., Chua, M. L., Zhang, C., Hong, S., Kumar, Y., Gokhale, R., & Ee, P. L. R. (2020).
Frontiers in Microbiology, 11, 417.
Ultra-short antimicrobial peptoids show propensity for membrane activity against multi-drug resistant mycobacterium tuberculosis.
Khara, J. S., Mojsoska, B., Mukherjee, D., Langford, P. R., Robertson, B. D., Jenssen, H., ... & Newton, S. M. (2020).
Nanoscale, 12(33), 17411-17425.
Nucleic acid peptide nanogels for the treatment of bacterial keratitis.
Obuobi, S., Mayandi, V., Nor, N. A. M., Lee, B. J., Lakshminarayanan, R., & Ee, P. L. R. (2020)
2008 - 2019
Patent. Novel polysaccharide-based hydrogel scaffolds for wound care, CA3137987A1. Chi Zhang, Shiqi HONG, Rajeev Gokhale, Pui Lai Rachel EE, Jian Yao NG. (2019) Journal of Controlled Release, 313, 120-130. Facile and efficient encapsulation of antimicrobial peptides via crosslinked DNA nanostructures and their application in wound therapy. Obuobi, S., Tay, H. K. L., Tram, N. D. T., Selvarajan, V., Khara, J. S., Wang, Y., & Ee, P. L. R. (2019). Journal of Global Antimicrobial Resistance, 17, 312-315. Do antimicrobial stewardship programme interventions reduce the rate of and protect against Clostridium difficile infection? Chia, B. Y., Teo, J. Q. M., Lee, W., Liew, Y. X., Ee, R. P. L., Chlebicki, M. P., ... & Kwa, A. L. H. (2019). Advanced Healthcare Materials, 2018, 7, e1701388. Phenylboronic Acid Functionalized Polycarbonate Hydrogels for Controlled Release of Polymyxin B in Pseudomonas Aeruginosa Infected Burn Wounds. Obuobi S., Voo Z.X., Low M.W., Czarny B., Selvarajan V., Ibrahim N.L., Yang Y.Y., Ee P.L.R. Antimicrobial agents and chemotherapy, 2018, 62, e00183 Rapid antibiotic combination testing for carbapenem-resistant Gram negative bacteria within 6h using adenosine triphosphate bioluminescence. Cai Y., Seah C.L., Leck H., Lim T.P., Teo J.Q., Lee W., Tan T.T., Koh T.H., Ee P.L.R., and Kwa A.L. Macromolecular Bioscience, 2018, e1800196. Antimicrobial and Anti‐Biofilm Activities of Surface Engineered Polycationic Albumin Nanoparticles with Reduced Hemolytic Activity. Obuobi S., Wang Y., Khara J., Riegger A., Kuan S.L. and Ee P.L.R. Infection and Drug Resistance, 2018, 11, pp. 1591. Integrated pharmacokinetic-pharmacodynamic modeling to evaluate empiric carbapenem therapy in bloodtsream infections. Lim T.P., Wang R., Poh G.Q., Koh T.H., Tan T., Lee W., Teo J.Q., Cai Y., Tan T.T., Ee P.L.R. and Kwa A.L. Advanced Healthcare Materials, 2018, 7, e1701388. Phenylboronic Acid Functionalized Polycarbonate Hydrogels for Controlled Release of Polymyxin B in Pseudomonas Aeruginosa Infected Burn Wounds. Obuobi S., Voo Z.X., Low M.W., Czarny B., Selvarajan V., Ibrahim N.L., Yang Y.Y., Ee P.L.R. Antimicrobial agents and chemotherapy, 2018, 62, e00183 Rapid antibiotic combination testing for carbapenem-resistant Gram negative bacteria within 6h using adenosine triphosphate bioluminescence. Cai Y., Seah C.L., Leck H., Lim T.P., Teo J.Q., Lee W., Tan T.T., Koh T.H., Ee P.L.R., and Kwa A.L. Macromolecular Bioscience, 2018, e1800196. Antimicrobial and Anti‐Biofilm Activities of Surface Engineered Polycationic Albumin Nanoparticles with Reduced Hemolytic Activity. Obuobi S., Wang Y., Khara J., Riegger A., Kuan S.L. and Ee P.L.R. Infection and Drug Resistance, 2018, 11, pp. 1591. Integrated pharmacokinetic-pharmacodynamic modeling to evaluate empiric carbapenem therapy in bloodtsream infections. Lim T.P., Wang R., Poh G.Q., Koh T.H., Tan T., Lee W., Teo J.Q., Cai Y., Tan T.T., Ee P.L.R. and Kwa A.L. Antimicrobial Resistance and Infection Control, 2017, 6 (1): Article no. 27. Candidemia in a major regional tertiary referral hospital - epidemiology, practice patterns and outcomes. Teo, J.Q., Candra, S.R., Lee, S.J., Chia, S.Y., Leck, H., Tan, A.L., Neo, H.P., Leow, K.W., Cai, Y., Ee, P.L.R., Lim, T.P., Lee, W., Kwa, A.L.H. Acta Biomaterialia, 2017, 15 (57): 103-114. Disruption of drug-resistant biofilms using de novo designed short α-helical antimicrobial peptides with idealized facial amphiphilicity. Khara, J.S., Obuobi, S., Wang, Y., Hamilton, M.S., Robertson, B.D., Newton, S.M., Yang, Y.Y., Langford, P.R., Ee, P.L.R. European Journal of Nutrition, 2017, 56 (1): 29-43. Understanding the cholesterol metabolism-perturbing effects of docosahexaenoic acid by gas chromatography–mass spectrometry targeted metabonomic profiling. Bahety, P., Nguyen, T. H. V., Hong, Y., Zhang, L., Chan, E.C.Y., Ee, P.L.R. Antibiotics, 2017, 6 (3): 14 Macromolecular conjugate and biological carrier approaches for the targeted delivery of antibiotics. Tram, N.D.T., Ee, P.L.R. Acta Biomaterialia, 2016, 46: 211-220. Biodegradable functional polycarbonate micelles for controlled release of amphotericin B. Wang, Y., Ke, X., Voo, Z.X., Yap, S.S.L., Yang, C., Gao, S., Liu, S., Venkataraman, S., Obuobi, S.A.O., Khara, J.S., Yang, Y.Y. & Ee P.L.R. Journal of Antimicrobial Chemotherapy, 2016, 71(8): 2181-2191. Unnatural amino acid analogues of membrane-active helical peptides with anti-mycobacterial activity and improved stability. Khara, J. S., Priestman, M., Uhía, I., Hamilton, M. S., Krishnan, N., Wang, Y., Yang Y. Y., Langford P. R., Newton S. M., Robertson B. D. & Ee, P.L.R. Journal of Enzyme Inhibition and Medicinal Chemistry, 2016, 31: 194-204. The role of modulation of antioxidant enzyme systems in the treatment of neurodegenerative diseases. Obuobi, S., Karatayev, S., Chai, C.L.L., Ee, P.L.R., & Mátyus, P. Antimicrobial Agents and Chemotherapy, 2016, 60(7): 4013-4022. Clinical efficacy of polymyxin monotherapy versus nonvalidated polymyxin combination therapy versus validated polymyxin combination therapy in extensively drug-resistant gram-negative Bacillus infections. Cai, B., Cai, Y., Liew, Y.X., Chua, N.G., Teo, J.Q.M., Lim, T.P., Kurup A., Ee P.L.R., Tan T.T., Lee W. & Kwa, A.L.H. Biomicrofluidics, 2016,10 (3): 034115. Stability and efficacy of synthetic cationic antimicrobial peptides nebulized using high frequency acoustic waves. Wang, Y., Rezk, A.R., Khara, J.S., Yeo, L.Y., & Ee, P.L.R. Biomaterials in Regenerative Medicine and the Immune System, 2015, 95-112. Nature-inspired multifunctional host defense peptides with dual antimicrobial-immunomodulatory activities. Khara, J.S., & Ee, P.L.R. Neurochemistry International, 2015, 87: 13-21. Wnt co-receptor LRP5/6 overexpression confers protection against hydrogen peroxide-induced neurotoxicity and reduces tau phosphorylation in SH-SY5Y cells. Zhang, L., Bahety, P., & Ee, P.L.R. Acta Biomaterialia, 2015, 28: 99-108. Designing α-helical peptides with enhanced synergism and selectivity against Mycobacterium smegmatis: Discerning the role of hydrophobicity and helicity. Khara, J.S., Lim, F.K., Wang, Y., Ke, X.Y., Voo, Z.X., Yang, Y.Y., Lakshminarayanan R. & Ee, P.L.R. Biomaterials, 2014, 35 (9): 3102-3109. Synthetic modifications of the immunomodulating peptide thymopentin to confer anti-mycobacterial activity. Wang, Y., Ke, X.Y., Khara, J.S., Bahety, P., Liu, S., Seow, S.V., Yang Y.Y. & Ee, P.L.R. PLoS ONE, 2014, 9 (2): e90123. Metabotyping of docosahexaenoic acid - Treated alzheimer's disease cell model. Bahety, P., Tan, Y.M., Hong, Y., Zhang, L., Chan, E.C.Y., & Ee, P.L.R. Biomaterials, 2014, 35 (6): 2032-2038. Anti-mycobacterial activities of synthetic cationic α-helical peptides and their synergism with rifampicin. Khara, J.S., Wang, Y., Ke, X.Y., Liu, S., Newton, S.M., Langford, P.R., Yang Y.Y. & Ee, P.L.R. European Journal of Medicinal Chemistry, 2014, 71: 67-80. Functionalized curcumin analogs as potent modulators of the Wnt/β-catenin signaling pathway. Leow, P.C., Bahety, P., Boon, C.P., Lee, C.Y., Tan, K.L., Yang, T., & Ee, P.L.R. Angewandte Chemie - International Edition, 2013, 52 (2): 674-678. Broad-spectrum antimicrobial and biofilm-disrupting hydrogels: Stereocomplex-driven supramolecular assemblies. Li, Y., Fukushima, K., Coady, D.J., Engler, A.C., Liu, S., Huang, Y., Cho J.S., Guo Y., Miller L.S., Tan J.P.K., Ee P.L.R., Fan W., Yang Y.Y. & Hedrick, J.L. Biomaterials, 2012, 3 (27): 6533-6541. Nanostructured PEG-based hydrogels with tunable physical properties for gene delivery to human mesenchymal stem cells. Li, Y., Yang, C., Khan, M., Liu, S., Hedrick, J. L., Yang, Y.Y., & Ee, P.L.R. ChemMedChem, 2012, 7 (9): 1567-1579. Curcumin Analogues with Potent and Selective Anti-proliferative Activity on Acute Promyelocytic Leukemia: Involvement of Accumulated Misfolded Nuclear Receptor Co-repressor (N-CoR) Protein as a Basis for Selective Activity. Tan, K.L., Koh, S.B., Ee, P.L.R,, Khan, M., & Go, M.L. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (51): 20778-20783. Notch1 regulates the expression of the multidrug resistance gene ABCC1/MRP1 in cultured cancer cells. Cho, S., Lu, M., He, X., Ee, P.L.R., Bhat, U., Schneider, E., Miele L. & Beck, W.T. Macromolecular Rapid Communications, 2011, 32 (22): 1826-1833. Novel biodegradable block copolymers of poly(ethylene glycol) (PEG) and cationic polycarbonate: Effects of PEG configuration on gene delivery. Yang, C., Ong, Z.Y., Yang, Y.Y., Ee, P.L.R., & Hedrick, J.L. Advanced Drug Delivery Reviews, 2011, 63 (14-15): 1228-1246. The effects of polymeric nanostructure shape on drug delivery. Venkataraman, S., Hedrick, J.L., Ong, Z.Y., Yang, C., Ee, P.L.R., Hammond, P.T., & Yang, Y.Y. ACS Nano, 2011, 5 (6): 4670-4678. Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. Nayak, T.R., Andersen, H., Makam, V.S., Khaw, C., Bae, S., Xu, X., Ee, P.L.R., Ahn J. H., Hong, B. H., Pastorin, G. & Özyilmaz, B. Current Opinion in Colloid and Interface Science, 2011, 16 (3): 182-194. Mixed micelles self-assembled from block copolymers for drug delivery. Ebrahim Attia, A.B., Ong, Z.Y., Hedrick, J.L., Lee, P.P., Ee, P.L.R., Hammond, P.T., & Yang, Y.Y. Journal of Controlled Release, 2011, 152 (1): 120-126. Rational design of biodegradable cationic polycarbonates for gene delivery. Ong, Z.Y., Fukushima,K., Coady, D.J., Yang, Y.Y., Ee, P.L.R., & Hedrick, J.L. Biomaterials, 2011, 32 (9): 2369-2378. The role of PEG architecture and molecular weight in the gene transfection performance of PEGylated poly(dimethylaminoethyl methacrylate) based cationic polymers. Venkataraman, S., Ong, W.L., Ong, Z.Y., Loo, S.C.J., Ee, P.L.R., & Yang, Y.Y. Investigational New Drugs, 2010, 28 (6): 766-782. Antitumor activity of natural compounds, curcumin and PKF118-310, as Wnt/β-catenin antagonists against human osteosarcoma cells. Leow, P.C., Tian, Q., Ong, Z.Y., Yang, Z., & Ee, P.L.R. Biomaterials, 2010, 31 (28): 7298-7307. Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage. Liu, S.Q., Tian, Q., Hedrick, J.L., Hui, J.H.P., Ee, P.L.R., & Yang, Y.Y. Nano Today, 2010, 5 (4): 296-312. Polymer- and lipid-based nanoparticle therapeutics for the treatment of liver diseases. Li, L., Wang, H., Ong, Z.Y., Xu, K., Ee, P.L.R., Zheng, S., Hedrick J.L. & Yang, Y.Y. Macromolecular Rapid Communications, 2010, 31 (13): 1148-1154. Injectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cells. Qiong Liu, S., Tian, Q., Wang, L., Hedrick, J.L., Po Hui, J.H., Yan Yang, Y., & Ee, P.L.R. Current Chemical Biology, 2010, 4 (1): 49-63. Natural compounds as antagonists of canonical Wnt/β-catenin signaling. Leow, P.C., Ong, Z.Y., & Ee, P.L.R. Current Nanoscience, 2010, 6 (2): 141-154. Crucial parameters responsible for carbon nanotubes toxicity. Nayak, T.R., Leow, P.C., Ee, P.L.R., Arockiadoss, T., Ramaprabhu, S., & Pastorin, G. Soft Matter, 2009, 6 (1): 67-81. Synthetic hydrogels for controlled stem cell differentiation. Liu, S.Q., Tay, R., Khan, M., Ee, P.L.R., Hedrick, J.L., & Yang, Y.Y. Biomaterials, 2009, 30 (8): 1453-1461. Biodegradable poly(ethylene glycol)-peptide hydrogels with well-defined structure and properties for cell delivery. Liu, S.Q., Ee, P.L.R., Ke, C.Y., Hedrick, J.L., & Yang, Y.Y. Biomacromolecules, 2009, 10 (1): 41-48. Efficient delivery of bcl-2-targeted siRNA using cationic polymer nanoparticles: Downregulating mRNA expression level and sensitizing cancer cells to anticancer drug. Beh, C.W., Seow, W.Y., Wang, Y., Zhang, Y., Ong, Z.Y., Ee, P.L.R., & Yang, Y.Y. European Journal of Pharmaceutical Sciences, 2008, 35 (4): 293-306. Dimethoxyaurones: Potent inhibitors of ABCG2 (breast cancer resistance protein). Sim, H.M., Lee, C.Y., Ee, P.L.R., & Go, M.L. European Journal of Pharmaceutical Sciences, 2008, 35 (1-2): 30-41. Modulation of breast cancer resistance protein (BCRP/ABCG2) by non-basic chalcone analogues. Han, Y., Riwanto, M., Go, M.L., & Ee, P.L.R.